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The linear range can be measured simply by making a plot of analyte concentration versus fluorescence, using evenly-spaced analyte concentrations, and seeing at what concentration the data deviate from a straight line that is tangent to the low end of the concentration range.
Determining the linear range is relatively easy, and can be achieved by taking a sample and performing a serial dilution. If the ranges overlap then determining the amount of sample to load is also similarly easy.
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